Variation in Metal Ion Concentrations in the Haemolymph of the Silkworm, Bombyx mori During Development
نویسندگان
چکیده
Changes in the levels of haemolymph metal ions of two binoltine races of the silkworm, Bombyx mori (NB4D2 and CSR2) and the multivoltine race (Pure Mysore ) was studied at different stages of larval and pupal development. The haemolymph metal ions showed significant changes during different stages of larval development like moult, post-moult, feeding and spinning and pupal stages like pre-pupa, pupa and pharate adult, in selected silkworm races. The larval haemolymph was characterized by low manganese and very high zinc and iron concentrations and moderately high copper ions. Haemolymph metal ions were relatively higher in NB4D2 and CSR2 than multivoltine Pure Mysore due to higher levels of ingestion in the bivoltine races. The haemolymph metal ions of bivoltine races were about 30% higher than the multivoltine Pure Mysore. Haemolymph zinc of pre-pupae showed a significant increase from that of spinning larvae. The zinc levels improved significantly during different stages of pupal development. Higher osmolar concentration of haemolymph of bivoltine races provides a favourable passive absorption gradient of digested nutrients in the lumen to haemolymph across midgut epithelium. The impact of races was significantly more than that of ontogeny in upward changes in haemolymph metal ions.
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